The conscious, resting heart rate and body temperature of healthy western lowland gorillas (Gorilla gorilla gorilla) is not reported in the literature. To obtain this information, two clinically healthy adult male western lowland gorillas in a training program were conditioned to allow auscultation and obtain tympanic temperatures. The mean heart rate was 73.8 ± 8.96 beats per minute (n = 176), and it was lower than mean heart rate reported in studies on anesthetized gorillas. The mean tympanic temperature was 35.5 ± 0.7°C (95.9 ± 1.23°F) (n = 209). The right tympanic temperature (rtt) and left tympanic temperature (ltt) were recorded separately for one gorilla. A small but statistically significant difference was present between the mean rtt of 35.8 ± 0.7°C (96.5 ± 1.2°F) (n = 137) and the mean ltt of 35.7 ± 0.8°C (96.2 ± 1.3° F) (n = 121).